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custom array  (Twist Bioscience)

 
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    Twist Bioscience custom array
    Custom Array, supplied by Twist Bioscience, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/custom+array/array+custom+gene/pmc12955154-243-11-9
    Average 86 stars, based on 1 article reviews
    custom array - by Bioz Stars, 2026-08
    86/100 stars

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    Determining assembly efficiency by gel electrophoresis, related to step 7 sgRNA library insert (∼140 bp), digested pLentiCRISPR_v2 backbone (∼13,000 bp), and HiFi assembly reaction product were resolved on a 2% TAE agarose gel. Electrophoresis was performed at 120 V for 30 min in 1× TAE buffer, followed by SYBR green staining and UV visualization. 1 Kb DNA ladder were included for size validation.

    Journal: STAR Protocols

    Article Title: In vivo CRISPR screening protocol to identify metastasis mediators using iteratively selected mouse models

    doi: 10.1016/j.xpro.2025.104042

    Figure Lengend Snippet: Determining assembly efficiency by gel electrophoresis, related to step 7 sgRNA library insert (∼140 bp), digested pLentiCRISPR_v2 backbone (∼13,000 bp), and HiFi assembly reaction product were resolved on a 2% TAE agarose gel. Electrophoresis was performed at 120 V for 30 min in 1× TAE buffer, followed by SYBR green staining and UV visualization. 1 Kb DNA ladder were included for size validation.

    Article Snippet: Synthesize the pooled sgRNA oligo library as a custom DNA array using a commercial high-throughput synthesis service (for instance, GeneWiz or GenScript).

    Techniques: Nucleic Acid Electrophoresis, Agarose Gel Electrophoresis, SYBR Green Assay, Staining, Biomarker Discovery